Performing a statistical test



The Bioinformatic Studio provides a large array of statistical tests. These tests are performed by the R software, which means it is necessary to have a local R installation.
  1. Select the AaDistribution result in the result table
  2. Select TTest in the Tests menu
A dialog will appear which offers all the switches and preferences for this test that are provided by the R software. The most important setting is the Dataselect field. There you have to specify which part of the result is used for the test. This has to be specified in R notation. For example we want to test if the distribution of an amino acid differs in the two data sets. The selected result in the result table can now be refered as data.
  1. Enter
    data[data$AA=="W" & data$DATASET=="setA",3],data[data$AA=="W" & data$DATASET=="setB",3]
    in the Dataselect field.
This setting uses selects the third column(Message/Data Window) of dataset setA where the amino acid is "W" (Thryptophan) and the same for dataset setB. This is the data for the t-test.
You can make additional settings which differ from for each test (click advanced to see all settings). The Help button shows a description of the test and explains all the available settings.
  1. Click OK to execute the test
Like a engine result, the test result will be shown in the result table and can accessed by double-click on the result or the popup menu (right mouse click).